Manual protein purification techniques, such as those based on the use of spin and gravity-flow columns, are burdened, in part, by the lack of standardized affinity tag purification protocols.

A list of companies offering complete affinity fusion protein-purification systems (vectors and resins) is shown in the accompanying table. Although the GST tag is more difficult to use than its ...

CEPI and POP BIO are committed to enabling equitable access to the outputs of their partnership, in line with CEPI’s ...

News Protein Purification Gets Easier Courtesy of Amersham BiosciencesResearchers who need to purify large numbers of His- or GST-tagged proteins take note: A new robotic system can speed up the ...

Understanding and characterizing complex compounds is essential for drug discovery and development. Chromatography is a vital ...

RAP Tag and PMab-2 Antibody: A Tagging System for Detecting and Purifying Proteins in Plant Cells. Frontiers in Plant Science, 2020; 11 DOI: 10.3389/fpls.2020.510444 ...

Figure 3 Methods Each system was run according to its respective purification and desalting protocols. Purified and desalted protein concentration was determined by SDS-PAGE with UV detection.