use RNA to guide them to specific sites on DNA. TIGR systems can be reprogrammed to target any DNA sequence of interest, and they have distinct functional modules that can act on the targeted DNA.

They also built a machine learning model to predict how well a guide RNA will perform for a set of target sequences, saving future users the headache of multiple trials. In its unaltered state, the ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

The type I CRISPR systems have some advantages in functionality, explains Osakabe, including longer guide RNA sequences and different mutation profiles. Typical guide RNAs are about 20 nucleotides ...

The natural RNAi mechanism can be directed by either short interfering RNA (siRNA ... the entire sequence pairs with the target strand with high complementarity. This often results in a 100 percent ...

Alternative splicing (AS) of pre-mRNA plays a crucial role in tissue-specific gene regulation, with disease implications due to splicing defects. Predicting and manipulating AS can therefore uncover ...

The Tas protein uses an RNA guide to recognize a specific target DNA sequence. A vast search of natural diversity has led scientists at MIT’s McGovern Institute and the Broad Institute of MIT ...