In gene editing tests, GEENIE was used to deliver CRISPR-Cas9/gRNA as ribonucleoprotein (RNP). Sanger sequencing was performed by a third party (GenOmbio) and analyzed with Synthego’s ICE ...

In the CRISPR-Cas9 gene editing system, the Cas9 nuclease is introduced into cells and directed to the location of interest with synthetic guide RNA (gRNA), where Cas9 cleaves the two DNA strands. Off ...

1 At conferences like Pittcon, where analytical chemistry and life science collide, CRISPR’s growing influence ... but without the guide RNA (gRNA), it would be adrift. The gRNA functions ...

The CRISPR-Cas (Clustered Regularly Interspaced ... have reduced off-target effects. 6 GC content in the gRNA sequence between 40% and 60% elevates on-target activity because higher GC content ...