For example, a knockout cell line for liver cancer might turn off a gene linked with tumor growth. Cancer isn’t directly passed down from parents to children the way some traits and health ...

In theory, mutating a gene of interest inside stem cells enables researchers to analyze the effects of that mutation on the development of particular cell types. In the laboratory of Jack Parent at ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

In particular, recent expression quantitative trait loci (eQTL) studies have highlighted POLDIP2 as a significant gene that confers ... on the POLDIP2 knockout cell line and showed that it ...

gene knockout and knock-in studies are typically performed on cancer cell lines to investigate the efficacy of an existing ...

Student should read about the project, and understand the basic concept of mouse genetics: Punnett's square, two genes distribution, cell- type specific Cre drivers, LoxP sites, Rosa26 locus, knock-in ...

Rebecca Roberts is a science writer and communicator. She earned her PhD in molecular biology from the University of the Sunshine Coast in Australia and completed a two-year postdoctoral fellowship at ...

The scientists analyzed datasets from DepMap's genome-wide CRISPR knockout screens in more than 1,200 cell lines. They noticed ... deletions within the FOCAD gene specifically caused the cells ...