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Addition of a robust fluorescent translocation assay requiring minimal sample ... Catanese, M., Law, L. et al. Real-time imaging of hepatitis C virus infection using a fluorescent cell-based ...
NRK cells that expressed both CD3δ fusion proteins and, as a control for cell permeabilization, red fluorescent protein (RFP), were subjected to the FPP assay.
A truncated strand of GFP is genetically fused to the bacterial protein of interest and the remainder of GFP is produced by mammalian cells. The individual components are nonfluorescent, but when ...
An efficient, robust method of examining the interactions between cells uses fluorescent tagging to simultaneously analyze multiple cell populations, speeding up a once tedious and limiting process.
To isolate white blood cells, 4mL of cord blood was lysed with red blood cell lysis buffer (BioLegend) in the dark for 20 minutes. Cells were then washed 2x in assay buffer. Cells were suspended in ...