Figure 2. The standard curves of DNA fragment. Image Credit: ACROBiosystems Quantification of plasmid DNA (pDNA) sample Three pDNA samples were tested using the E. coli qPCR assay. In these cases ...

No endo-free plasmid purification or cleanup methods were used. Plasmid yields for ClearColi K-12 and DH10B were very similar, with 0.4 to 0.5 μg of DNA recovered per mL culture per OD600 unit.

The success of E. coli bacteria depends on their ability to multiply very rapidly by dividing into new cells. The bacteria can divide as quickly as they can make an entire new copy of their DNA while ...