Figure 2. The standard curves of DNA fragment. Image Credit: ACROBiosystems Quantification of plasmid DNA (pDNA) sample Three pDNA samples were tested using the E. coli qPCR assay. In these cases ...

No endo-free plasmid purification or cleanup methods were used. Plasmid yields for ClearColi K-12 and DH10B were very similar, with 0.4 to 0.5 μg of DNA recovered per mL culture per OD600 unit.

Escherichia coli (E. coli) has been commonly used for the production of biopharmaceuticals. Among the impurities that must be monitored in biopharmaceuticals is residual host-cell DNA (HCD). This ...