IT is known that many proteolytic enzymes—trypsin, chymotrypsin, thrombin, papain and others—hydrolyse peptide, amide and ester bonds. Pepsin, however, is generally believed to hydrolyse the ...

Glutamine (in the activated cyanomethyl ester form) was chosen because its amide side chain could be used for specific binding through hydrogen bond contacts with the selected RNA. With this ...