Enzyme–substrate complexes can no longer form as the substrates no longer fit into the active site. The enzyme is denatured.

Changes in pH also alter the shape of an enzyme’s active site. Each enzyme work bests at a specific pH value. The optimum pH for an enzyme depends on where it normally works. For example ...

Exo Therapeutics raised $78 million in series B financing to develop a new strategy for drugging enzymes. Many drugs work by blocking target enzymes’ active sites, which perform reactions like ...

The “lock and key” model was first proposed in 1894. In this model, an enzyme’s active site is a specific shape, and only the substrate will fit into it, like a lock and key.

R. Rogers Yocum, David J. Waxman, James R. Rasmussen, Jack L. Strominger, Mechanism of Penicillin Action: Penicillin and Substrate Bind Covalently to the Same Active Site Serine in Two Bacterial ...