Figure 7. Different E. coli strains transformed with a positive control plasmid. The transformation protocol can be used to effectively transform different strains of E. coli cells with plasmid DNA.

New research has made encouraging progress in tackling not one but two of the biggest problems facing our planet right now: ...

This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare high-efficiency transformation competent E. coli; (iii) generation of double ...

We have developed a screen for detecting E. coli colonies that produce soluble ... soluble protein can easily be identified. This protocol can be used to screen thousands of constructs in a ...

The 1 million base pairs chromosome can be used for E. coli transformation. This E. coli-based technology has the potential to greatly advance synthetic genomics. The issue of concern was that the ...