Procedure Protein X was expressed in E. coli from a plasmid under the control of a T7 promoter and the gene of interest. A 100 mL overnight culture was grown at 37ºC. To scale up, 16 hours later ...

Cell lysis─by sonication or bead beating, for example─is a key step in preparing extracts for cell-free expression systems. To create high protein-production capacity extracts, standard practice is to ...

Researchers have developed a genome-scale model that can accurately predict how E. coli bacteria respond to temperature changes and genetic mutations. The work sheds light on how cells adapt under ...

Hence, the design of a temperature-controlled cell-free protein synthesis (tcCFPS) system based on E. coli was carried out with the repressor cI protein in this study. The corresponding tcCFPS ...

Letter Published: 21 July 1962 Polyribonucleotide-directed Protein Synthesis using an E. coli Cell-free System M. S. BRETSCHER & M. GRUNBERG-MANAGO Nature 195, 283–284 (1962) Cite this article ...

Sometimes the simplest expression platform is the best (or only) platform, says AbSci, which engineers Escherichia coli to generate high-titer, properly folded, and easy-to-purify biologics.

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed ...