The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need ... separately transformed into the BL21(DE3) expression host.

Expression was performed in E. coli BL21 Star (DE3) on LB plates with 50 µg/mL kanamycin. Recombinant proteins were purified using Strep-Tactin affinity chromatography, and concentrations ...