However, observations carried out with a phase-contrast microscope on isolated living liver cells from animals kept in hypoxia indicate that vacuoles are not visible in non-fixed, unstained cells.

Digital holographic ... for time-lapse microscopy, high-throughput screening, and diagnostics. The scheme enables the quantitative monitoring of the dynamical behaviour of cells with nanometre ...

These are liver cells under microscopy. At the top, in a healthy liver, the liver cells are organized normally. At the bottom, an accumulation of fat (white circles) is visible.