Pre-activated PBMCs (anti-CD3/IL2, 4 d) were used for incubating A549 CytoLight™ Red (adenocarcinoma) cells (5K/well) in the presence of Opti-Green and CD8 or CD45 Abs labeled with FabFluor-488.

Myeloma MRD flow cytometry requires at least two markers for plasma cell identification (CD38 and either CD45 or CD138, preferably all three) and some combination of several additional markers to ...

Method validation To validate the results, the researchers performed microscopy along with flow cytometry for ten samples and tested the levels of IL-2, IFN-y, and CD45.

Reference Guo J, Wang H, Xiong S, et al. Simplified flow cytometry scoring for diagnosis and prognosis of myelodysplastic symptom. Am J Transl Res. 2020;12 (11):7449-7458.