The Cas9 endonuclease is guided by a single guide RNA (sgRNA) to a specific DNA sequence, where it creates a double-strand break. This break can then be repaired through either non-homologous end ...

For example, the PARP1 homology-directed repair (HDR) plasmid exploits the HDR pathway that enables precise DNA repair at the location of the CRISPR/Cas9 DSB site according to a targeted repair ...

and the Cas9 protein, which induces a DNA double-strand break (DSB) 2. Nuclease-induced DNA DSBs can be repaired through ...

(B, C) Confocal microscopic images of coronal brain sections of the hippocampus of Cas9 mice, showing the EGFP ... Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems ...

Danielle is a Senior Editor at The Scientist. She has a background in neuroscience. Her science communication experience spans journalism, scientific publishing, and science education and outreach.