Researchers at the Max Planck Institute for Evolutionary Anthropology have addressed a longstanding challenge in CRISPR ...

In the CRISPR-Cas9 gene editing system, the Cas9 nuclease is introduced into cells and directed to the location of interest with synthetic guide RNA (gRNA), where Cas9 cleaves the two DNA strands. Off ...

The team demonstrated that NovaIscB is a good scaffold for a variety of genome editing tools. "It biochemically functions very similarly to Cas9, and that makes it easy to port over tools that were ...

CRISPR/Cas9 screens require the delivery of a pool of guide RNAs (gRNA) and Cas9 nuclease into a cell line. The most common and successful delivery approach involves using an “all-in-one” lentiviral ...

The method involves introducing Cas9 nuclease—an enzyme that cuts DNA—into cells, with a synthetic guide RNA (gRNA) that directs the enzyme to the required location. The ability to cut the ...

"Our study shows that, by better understanding the CRISPR/Cas9 protein and its gRNA component, we can more accurately hit and cut the DNA and thereby optimise the effectiveness of gene ...

Scientists in the lab of Nobel Prize winner Jennifer Doudna, PhD, created enveloped delivery vehicles (EDVs) that can carry Cas9 and gRNA proteins for precise genome editing in living organisms.

A new journal paper published late last week revealed that a “cutting” element of the CRISPR-Cas9 gene-editing technique could be under threat from the body’s own immune system. The paper ...