Introduction: Why CRISPR Screening Still Feels Slow CRISPR-Cas9 technology has revolutionized gene editing, but biotech teams ...

CRISPR-Cas9 gene editing technology is widely used to to help study genes of interest and modify disease-associated genes. However, the system is associated with adverse effects, including ...

In Ba/F3 cell lines, sgRNA-mediated SHOC2 knockout led to pronounced cell lethality, with NRAS (Q61*) mutants exhibiting heightened sensitivity compared to KRAS (G12*)-mutant lines.

A restricted CRISPR screen can also be used for drug discovery purposes, in which a specific subset of genes that are relevant to the disease phenotype are explored.

Researchers utilize the CRISPR-Cas9 system to induce the synthesis of dopamine (DA) in the brains of a rat model for Parkinson’s disease.