Researchers at the Max Planck Institute for Evolutionary Anthropology have addressed a longstanding challenge in CRISPR ...

"Our study shows that, by better understanding the CRISPR/Cas9 protein and its gRNA component ... This model makes it possible for researchers to design gRNA components that can increase the ...

To this end, Thermo Fisher Scientific has built several software solutions to improve the usability of the CRISPR-Cas9 system by addressing two major challenges; the design of gRNAs and corresponding ...

"By combining our own data with publicly available data and including knowledge on the molecular interactions between gRNA, DNA and the CRISPR-Cas9 protein, we have succeeded in developing a ...

In contrast, with arrayed screens, experimenters know which gRNA was used in a particular ... The company has also developed a CRISPR-Cas9 library design tool that chooses gRNAs with high selectivity.

In the CRISPR-Cas9 gene editing system, the Cas9 nuclease is introduced into cells and directed to the location of interest with synthetic guide RNA (gRNA), where Cas9 cleaves the two DNA strands. Off ...

the team synthesized a library of 41,630 pairs of different gRNA and target DNA sequences. They studied them in a range of genetic scenarios using different CRISPR-Cas9 reagents to analyze how the ...

Improve DNA transfection efficiency with nuclear transfer: The delivery of Cas9 and gRNA ... the CRISPR substrates into the cells. It offers higher efficiencies, but it is laborious to design.

In order to be successful with CRISPR-Cas9 genome engineering, there are key considerations that need to be addressed, from the cell line you want to use, to guide design and screening for the ...