The ANCA staining pattern (cytoplasmatic or perinuclear) was assessed by indirect immunofluorescence. ANCA specificity for proteinase 3 (PR3, Organtec) or myeloperoxidase (MPO, Euroimmun) was measured ...

Antinuclear autoantibodies (ANAs) are important markers to diagnose autoimmune diseases, very serious and also invalidating illnesses. The benchmark procedure for ANAs diagnosis is the indirect ...

Immunofluorescence detected ANCA in 160 (89%) patients (cytoplasmic ANCA staining [c-ANCA] in 136 patients, perinuclear ANCA staining [p-ANCA] in 24 patients). Direct ELISA detected proteinase 3 ...

It is often perinuclear staining pattern (p)-ANCA positive. Yearly incidence is between one and three per million, with peak incidence in the fourth decade of life and there is a roughly equal sex ...

But in ANCA vasculitis, complements are normal, and the kidney biopsy does not have any staining, and we call it Pauci immune disease because we do not see complement deposition.