Two-dimensional polyacrylamide gel electrophoresis, or 2D PAGE, was introduced in 1975 concurrently by Joachim Klose 7 and Patrick H. O’Farrel. 8 In the first dimension, proteins are separated ...

When performing 1D and 2D gel electrophoresis within your laboratory, you doubtlessly pay a great deal of attention to your experimental design, sample preparation, and technical execution, likely ...

Analyze proteins better with these tips. Two-dimensional gel electrophoresis (2DE) is a uniquely powerful technique that allows separation of up to thousands of proteins while retaining pI and MW ...

Analyzing 2D-gel electrophoresis images remains a challenging task. Amongst the respective stages of the analysis, detection and segmentation of each individual spot are the most crucial. The ...

Spot-Segmentation, an essential stage of processing 2D gel electrophoresis images, remains a challenging process. The available software programs and techniques fail to separate overlapping protein ...

Microfluidic systems have taken over from two-dimensional (2D) gel electrophoresis techniques in some areas of protein separation and analysis, but the established methods are far from dead. Even ...

Proteomic analysis by 2D gel electrophoresis (2-DE) with immobilized pH gradients is in turn the best approach to large-scale protein-expression screening. Spots visualization is pivotal for ...

Gothenburg, Sweden, 11 October 2011: Denator AB announced today the release of a new kit to ensure scientists obtain a consistent, representative picture of protein profiles when analyzing ...

Gel electrophoresis is a fundamental technique in laboratories across the biological disciplines, permitting the separation of macromolecules such as DNA, RNA and proteins. Different separation media ...