Although DNA Pol θ depletion did not affect origin density, the authors found genome-wide changes in replication timing (both early to late and late to early) in a subset of replication domains ...

To better understand how the mismatches are removed during high-fidelity DNA replication, we determined the cryo-EM structure of the E. coli PolIIIα–exonuclease–θ–clamp complex bound to a ...