The basics of CAR T design and challenges in immunotherapy of solid ...
WEBMar 8, 2017 — In CAR T system, the key component is CAR construct. The main structure of CAR contains a single chain fraction variable (scFv) and a signaling pathway molecule, TCR zeta chain. The scFv contains an antigen binding site of heavy and light chain of antibody through a linker.
Engineering strategies to overcome the current roadblocks in CAR T …
WEBDec 17, 2019 — In this Review, we provide an overview of basic CAR design and discuss emerging strategies to engineer safer and more effective CAR T cells for the treatment of haematological and solid...
CAR-T cell manufacturing: Major process parameters and next …
WEBSep 3, 2023 — In this review, we discuss the major process parameters of autologous CAR-T cell manufacturing, as well as regulatory considerations and ongoing developments that will enable the next generation of CAR-T cell therapies.
Making Potent CAR T Cells Using Genetic Engineering and …
WEBJun 29, 2021 — To overcome the obstacles of CAR T cell therapy, such as poor activity in solid tumors and severe side effects caused by activated CAR T cells, the current CAR constructs have been modified in multiple ways to express specific genes or to deplete immune-suppressive genes.
Activation-inducible CAR expression enables precise control over ...
WEBJun 5, 2023 — We manufactured activation-inducible CAR T cells with the help of CRISPR-mediated targeted integration. This new type of engineered T cells expresses the CAR gene dependent on their activation...
Engineering the next-generation of CAR T-cells with CRISPR-Cas9 …
WEBMar 18, 2022 — Chimeric Antigen Receptor (CAR) T-cells represent a breakthrough in personalized cancer therapy. In this strategy, synthetic receptors comprised of antigen recognition, signaling, and costimulatory domains are used to reprogram T-cells to target tumor cells for destruction.
CAR-T design: Elements and their synergistic function
WEBMay 6, 2020 — We provide a comprehensive review of recent research on CAR design and systematically evaluate design aspects of the four major modules of CAR structure: the ligand-binding, spacer, transmembrane, and cytoplasmic domains, elucidating design strategies and principles to guide future immunotherapeutic discovery.
CAR-T cell potency: from structural elements to vector backbone ...
WEBSep 19, 2022 — In the current review, we will discuss how the molecular components of CAR construct and elements of lentiviral vector backbone plasmid transferring CAR expression cassette can contribute to CAR-T cell therapy success or failure. A schematic picture of the structural elements of six FDA-approved CAR-T products.
CRISPR/Cas-based CAR-T cells: production and application
WEBMay 31, 2024 — Allogeneic CAR-T cells from healthy donors offer blast-free products but risk graft-versus-host disease (GvHD) and rejection. We developed CD7-targeting CAR-T cells (RD13-01) from healthy donors with genetic modifications for fratricide resistance, GvHD prevention, and enhanced antitumor function.
CAR T Cell Therapy: A Versatile Living Drug - PMC - National …
WEBMar 27, 2023 — Brought about by an ever-growing understanding of cellular engineering, CAR T cells are T lymphocytes genetically modified with an appropriate DNA construct, which endows them with expression of a CAR, a fusion protein between a ligand-specific recognition domain, often an antibody-like structure, and …